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The present invention further extends to a pharmaceutical composition comprising the microemulsion and an active agent wherein the pharmaceutical composition is suitable for topical application on a subject. The present invention further provides a method suitable for the transdermal delivery of active agents in a range of therapeutic, cosmetic and research applications. The method comprises (a) preparing a water phase, including dissolving one or more selected active agents in water; (b) preparing an oil phase; (c) emulsifying the water phase and the oil phase using one or more zwitterionic surfactants and one or more co-surfactants, thereby forming a topical pharmaceutical composition; and (d) applying the topical pharmaceutical composition on a subject. The microemulsion of the present invention is useful inter alia for the topical delivery of anti-inflammatory agents, such as small molecules, proteins, antibodies, Fc fusion molecules and soluble receptors; vaccines; imaging agents; anti-cancer agents, particularly anti-melanoma agents; skin lightening including whitening agents; UV blocking agents, agents to reduce or nullify nitric oxide, agents useful for the treatment of erectile dysfunction; agents for increasing local circulation, agents for the amelioration of facial and skin ageing, agents for the treatment of obesity, re-pigmentation agents; genetic molecules such as RNAi, DNA, virosomes and anti-sense RNA; and agents useful in treating muscle dystrophy. The microemulsions have application in human and veterinary medicine as well as for agricultural animals. A list of agents contemplated for use in the microemulsions includes but is not limited to the list provided in Table 1.

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